Aquaporins (AQPs) are a recently discovered family of proteins that function as transmembrane water channels. These proteins regulate the delicate osmotic balance across the cell plasma membrane. Given that osmotic damage is the major contributing factor to cell death during freezing, we hypothesized that regulation of AQPs may have an unrealized role in protecting cells from osmotic damage during cryopreservation. Rat kidney inner medullar collecting duct (IMCD) cells were treated with arginine vasopressin (AVP) to increase the amount of AQP₂ in the external plasma membrane before freezing in University of Wisconsin solution at −4° C for 24 h. This resulted in a significant increase in cell viability on warming. Conversely, treatment of IMCD cells with AVP and W7 (which inhibits AQP₂ protein trafficking to the plasma membrane) before freezing resulted in a 55% decrease in cell viability. These preliminary data indicate that regulation of AQP₂ can attenuate cold-induced osmotic damage in rat kidney IMCD cells.